Electrophoretic studies on the phosphorylase isozymes.
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چکیده
منابع مشابه
Comparative Studies on Glycogen Phosphorylase
The isolation and crystallization of both forms of glycogen phosphorylase from human skeletal muscle (1) have made possible a detailed study of the properties of these enzymes in comparison with those of rabbit skeletal muscle (2, 3). Phosphorylase b and a from both species have been shown to crystallize under similar conditions and to have similar pH optima, electrophoretic mobilities, sedimen...
متن کاملStudies on Phosphorylase Activation in the Heart.
It is well known that stimulation of the heart by epinephrine and other sympathomimetic amines causes activation of glycogen phosphorylase, i.e. conversion of phosphorylase b to phosphorylase a, either in the intact animal or in the isolated organ (l-5). This reaction appears to be analogous to that occurring in skeletal muscle (6-8) and is catalyzed by phosphorylase b kinase. In skeletal muscl...
متن کاملElectrophoretic studies on human serum.
Archibald, R. M. (1944). J. biol. Chem. 154, 643. Coulson, R. A., Ellinger, P. & Holden, M. (1944). Biochem. J. 38, 150. Ellinger, P. (1946a). Biochem. J. 40, xxxi. Ellinger, P. (1946b). Biochem. J. 40, xxxii. Ellinger, P. & Coulson, R. A. (1944). Biochem. J. 38, 265. Ellinger, P., Fraenkel, G. & Abdel Kader, M. M. (1947). Biochem. J. 41, 559. Johnson, B. C. (1945). J. biol. Chem. 159, 227. Kon...
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These experiments were made to obtain more information about the surface of the red blood cell. Since "surface" means different things to different people, we shall define our meaning now. Strictly speaking, the surface studied by electrophoresis is the surface of shear between the cell (moving in the electric field) and the surrounding medium, for it is the potential at this surface which is t...
متن کاملElectrophoretic analysis of hemoglobin and isozymes in individual vertebrate cells.
Electrophoretic patterns of enzymes in single vertebrate erythrocytes may be visualized with the use of capillary tube polyacrylamide columns and stains which amplify the enzymatic activity. The following points have thereby been demonstrated:1. Peripheral blood of metamorphosing bullfrogs (Rana catesbeiana) contains two molecular forms of hemoglobin: embryonic (tadpole) and adult (frog). Only ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1975
ISSN: 0022-1554,1551-5044
DOI: 10.1177/23.10.1194664